Breivik, who is kept away from other inmates in a three-room cell, claimed being held in solitary confinement in “inhumane” conditions had seriously damaged him and made him even more radical in his far-right views.
But the country’s supreme court has upheld a ruling made by the court of appeal in January, and said there was “no basis” for a different conclusion.
Norwegians have derided the 38-year-old extremist over his complaints of cold coffee and microwave meals at Skien prison being “worse than waterboarding”.
Following the latest legal decision, Breivik’s lawyer, Oystein Storrvik, said he would take the case to the European Court of Human Rights in Strasbourg.
Mr Storrvik said: “We’ve always been prepared for the possibility that our case before the Norwegian courts may not succeed.”
Victims and survivors’ families have welcomed the supreme court’s decision.
Lisbeth Kristine Roeyneland, whose 18-year-old daughter Synne was killed in Breivik’s rampage, said: “I am very happy. This is very good news and shows that our justice system is working.”
She added: “This ensures that we are not going to see the terrorist for many, many years.”
Eskil Pedersen, who survived the shooting, simply said on Twitter: “Good.”
Last year, the country’s government had appealed a lower court ruling that Breivik’s isolation behind bars violated his rights.
He also hit out at regularly being strip-searched and handcuffed during the early part of his detention.
Breivik is currently serving a 21-year jail term – which can be extended indefinitely – for killing 77 people in a bomb and gun attack in 2011.
Eight lost their lives in a bombing in Oslo, while 69 others, including many teenagers, were shot dead at a meeting of young political activists on Utoya island.